(DNA 配列の) クロマトグラムの画像を作成しました。
各ベースコールに番号を付けるにはどうすればよいですか?
写真のピクセル長と塩基数を取得できます。これらを割ると、ベースコール間の平均距離が得られ、各ベースコールがどこに行くかの適切な概算が得られます。
写真の上部に数字 (1 - [塩基数]) を配置するにはどうすればよいですか?
GUI の図とプログラムの外観は次のとおりです。
https://drive.google.com/file/d/0B5nGPfKaY21KVFVsaE9XSHJmWG8/view?usp=sharing
ソースコードは次のとおりです。
[getChrom ボタンが押されると、renderTrace メソッドが実行され、トレースがレンダリングされます。]
public class TraceRender extends javax.swing.JFrame {
public TraceRender() {
initComponents();
}
/**
* This method is called from within the constructor to initialize the form.
* WARNING: Do NOT modify this code. The content of this method is always
* regenerated by the Form Editor.
*/
@SuppressWarnings("unchecked")
// <editor-fold defaultstate="collapsed" desc="Generated Code">
private void initComponents() {
fileChooser = new javax.swing.JFileChooser();
textarea = new javax.swing.JTextField();
getChrom = new javax.swing.JButton();
ScrollPane = new javax.swing.JScrollPane();
Label = new javax.swing.JLabel();
text = new javax.swing.JTextField();
Menu = new javax.swing.JMenuBar();
File = new javax.swing.JMenu();
Open = new javax.swing.JMenuItem();
Exit = new javax.swing.JMenuItem();
Edit = new javax.swing.JMenu();
Documentation = new javax.swing.JMenu();
Help = new javax.swing.JMenu();
fileChooser.setDialogTitle("This is my open dialog");
fileChooser.setFileFilter(new myCustomFilter());
setDefaultCloseOperation(javax.swing.WindowConstants.EXIT_ON_CLOSE);
textarea.setEditable(false);
textarea.setText("ABSOLUTE PATH");
getChrom.setText("Execute");
getChrom.addActionListener(new java.awt.event.ActionListener() {
public void actionPerformed(java.awt.event.ActionEvent evt) {
getChromActionPerformed(evt);
}
});
Label.setText("[AVI]-Error.noImageLoaded");
ScrollPane.setViewportView(Label);
text.setText("nothing");
File.setText("FILE");
Open.setText("Get File");
Open.addActionListener(new java.awt.event.ActionListener() {
public void actionPerformed(java.awt.event.ActionEvent evt) {
OpenActionPerformed(evt);
}
});
File.add(Open);
Exit.setText("Close Program");
Exit.addActionListener(new java.awt.event.ActionListener() {
public void actionPerformed(java.awt.event.ActionEvent evt) {
ExitActionPerformed(evt);
}
});
File.add(Exit);
Menu.add(File);
Edit.setText("EDIT");
Menu.add(Edit);
Documentation.setText("DOCUMENTATION");
Menu.add(Documentation);
Help.setText("HELP");
Menu.add(Help);
setJMenuBar(Menu);
javax.swing.GroupLayout layout = new javax.swing.GroupLayout(getContentPane());
getContentPane().setLayout(layout);
layout.setHorizontalGroup(
layout.createParallelGroup(javax.swing.GroupLayout.Alignment.LEADING)
.addGroup(layout.createSequentialGroup()
.addContainerGap()
.addComponent(textarea, javax.swing.GroupLayout.PREFERRED_SIZE, 1005, javax.swing.GroupLayout.PREFERRED_SIZE)
.addPreferredGap(javax.swing.LayoutStyle.ComponentPlacement.RELATED, 79, Short.MAX_VALUE)
.addComponent(getChrom, javax.swing.GroupLayout.PREFERRED_SIZE, 291, javax.swing.GroupLayout.PREFERRED_SIZE)
.addGap(49, 49, 49))
.addComponent(ScrollPane, javax.swing.GroupLayout.Alignment.TRAILING)
.addGroup(layout.createSequentialGroup()
.addGap(33, 33, 33)
.addComponent(text, javax.swing.GroupLayout.PREFERRED_SIZE, 630, javax.swing.GroupLayout.PREFERRED_SIZE)
.addContainerGap(javax.swing.GroupLayout.DEFAULT_SIZE, Short.MAX_VALUE))
);
layout.setVerticalGroup(
layout.createParallelGroup(javax.swing.GroupLayout.Alignment.LEADING)
.addGroup(layout.createSequentialGroup()
.addContainerGap()
.addGroup(layout.createParallelGroup(javax.swing.GroupLayout.Alignment.BASELINE)
.addComponent(textarea, javax.swing.GroupLayout.PREFERRED_SIZE, javax.swing.GroupLayout.DEFAULT_SIZE, javax.swing.GroupLayout.PREFERRED_SIZE)
.addComponent(getChrom))
.addGap(48, 48, 48)
.addComponent(ScrollPane, javax.swing.GroupLayout.PREFERRED_SIZE, 265, javax.swing.GroupLayout.PREFERRED_SIZE)
.addGap(53, 53, 53)
.addComponent(text, javax.swing.GroupLayout.PREFERRED_SIZE, javax.swing.GroupLayout.DEFAULT_SIZE, javax.swing.GroupLayout.PREFERRED_SIZE)
.addContainerGap(113, Short.MAX_VALUE))
);
pack();
}// </editor-fold>
private void OpenActionPerformed(java.awt.event.ActionEvent evt) {
// TODO add your handling code here:
int returnVal = fileChooser.showOpenDialog(this);
if (returnVal == JFileChooser.APPROVE_OPTION) {
File file = fileChooser.getSelectedFile();
textarea.setText(String.valueOf(file.getAbsolutePath()));
} else {
System.out.println("File access cancelled by user.");
}
}
private void ExitActionPerformed(java.awt.event.ActionEvent evt) {
// TODO add your handling code here:
System.exit(0);
}
private void getChromActionPerformed(java.awt.event.ActionEvent evt) {
try {
// TODO add your handling code here:
renderTrace();
} catch (IOException | UnsupportedChromatogramFormatException ex) {
Logger.getLogger(TraceRender.class.getName()).log(Level.SEVERE, null, ex);
}
}
private void renderTrace() throws IOException, UnsupportedChromatogramFormatException {
ABIFChromatogram abiChrom = new ABIFChromatogram();
File abi = new File(textarea.getText());
ABITrace abiTrace = new ABITrace(abi);
ABIFParser abiParse = new ABIFParser(abi);
ChromatogramFactory chromFactory = new ChromatogramFactory();
Chromatogram chrom = ChromatogramFactory.create(abi);
ChromatogramGraphic gfx = new ChromatogramGraphic(chrom);
gfx.setHeight(240);
gfx.setHorizontalScale(2.0f);
// set some options that affect the output
// turn off filled-in "callboxes"
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_A,
Boolean.FALSE);
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_C,
Boolean.FALSE);
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_G,
Boolean.FALSE);
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_T,
Boolean.FALSE);
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_OTHER,
Boolean.TRUE);
gfx.setOption(ChromatogramGraphic.Option.DRAW_CALL_SEPARATORS,
Boolean.TRUE);
// this option controls whether each trace/callbox/etc is scaled/positioned
// individually, or whether the scaling is done on all shapes at the level
// of the graphics context
// enabling this option is recommended for higher-quality output
gfx.setOption(ChromatogramGraphic.Option.USE_PER_SHAPE_TRANSFORM,
Boolean.TRUE);
BufferedImage bi = new BufferedImage(
gfx.getWidth(),
gfx.getHeight(),
BufferedImage.TYPE_INT_RGB);
Graphics2D g2 = bi.createGraphics();
g2.setBackground(Color.white);
g2.clearRect(0, 0, bi.getWidth(), bi.getHeight());
if (g2.getClip() == null) {
g2.setClip(new Rectangle(0, 0, bi.getWidth(), bi.getHeight()));
}
g2.setRenderingHint(RenderingHints.KEY_ANTIALIASING, RenderingHints.VALUE_ANTIALIAS_ON);
// the main event
gfx.drawTo(g2);
// work-around an OS X bug where sometimes the last Shape drawn
// doesn't show up in the output
g2.draw(new java.awt.Rectangle(-10, -10, 5, 5));
text.setText(String.valueOf(gfx.getWidth()));
ImageIcon ii = new ImageIcon(bi);
Label.setIcon(ii);
/*
try {
ImageIO.write(bi, "png", new File("gfx-image.png"));
} catch (IOException ex) {
ex.printStackTrace();
}
*/
}
/**
* @param args the command line arguments
*/
public static void main(String args[]) {
/* Set the Nimbus look and feel */
//<editor-fold defaultstate="collapsed" desc=" Look and feel setting code (optional) ">
/* If Nimbus (introduced in Java SE 6) is not available, stay with the default look and feel.
* For details see http://download.oracle.com/javase/tutorial/uiswing/lookandfeel/plaf.html
*/
try {
for (javax.swing.UIManager.LookAndFeelInfo info : javax.swing.UIManager.getInstalledLookAndFeels()) {
if ("Nimbus".equals(info.getName())) {
javax.swing.UIManager.setLookAndFeel(info.getClassName());
break;
}
}
} catch (ClassNotFoundException ex) {
java.util.logging.Logger.getLogger(TraceRender.class.getName()).log(java.util.logging.Level.SEVERE, null, ex);
} catch (InstantiationException ex) {
java.util.logging.Logger.getLogger(TraceRender.class.getName()).log(java.util.logging.Level.SEVERE, null, ex);
} catch (IllegalAccessException ex) {
java.util.logging.Logger.getLogger(TraceRender.class.getName()).log(java.util.logging.Level.SEVERE, null, ex);
} catch (javax.swing.UnsupportedLookAndFeelException ex) {
java.util.logging.Logger.getLogger(TraceRender.class.getName()).log(java.util.logging.Level.SEVERE, null, ex);
}
//</editor-fold>
/* Create and display the form */
java.awt.EventQueue.invokeLater(new Runnable() {
public void run() {
new TraceRender().setVisible(true);
}
});
}
class myCustomFilter extends javax.swing.filechooser.FileFilter {
@Override
public boolean accept(File file) {
// Allow only directories, or files with ".txt" extension
return file.isDirectory() || file.getAbsolutePath().endsWith(".ab1");
}
@Override
public String getDescription() {
// This description will be displayed in the dialog,
// hard-coded = ugly, should be done via I18N
return "ABIF Files (*.ab1)";
}
}
// Variables declaration - do not modify
private javax.swing.JMenu Documentation;
private javax.swing.JMenu Edit;
private javax.swing.JMenuItem Exit;
private javax.swing.JMenu File;
private javax.swing.JMenu Help;
private javax.swing.JLabel Label;
private javax.swing.JMenuBar Menu;
private javax.swing.JMenuItem Open;
private javax.swing.JScrollPane ScrollPane;
private javax.swing.JFileChooser fileChooser;
private javax.swing.JButton getChrom;
private javax.swing.JTextField text;
private javax.swing.JTextField textarea;
// End of variables declaration
}