pipeline - snakemake は常に " MissingOutputException を 44 行目で報告します。5 秒後にファイルが見つかりません:

Question

私の RNAs-seq パイプラインでは、snakemake によって常に同じエラー レポートが表示されます。

MissingOutputException in line 44 of /root/s/r/snakemake/my_rnaseq_data/Snakefile:
Missing files after 5 seconds:
03_align/wt2.bam
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.

ここに私のSnakefileがあります：

SBT=["wt1","wt2","epcr1","epcr2"]

rule all:
    input:
        expand("02_clean/{nico}_1.paired.fq", nico=SBT),
        expand("02_clean/{nico}_2.paired.fq", nico=SBT),
        expand("03_align/{nico}.bam", nico=SBT)

rule trim:
    input:
        "01_raw/{nico}_1.fastq",
        "01_raw/{nico}_2.fastq"
    output:
        "02_clean/{nico}_1.paired.fq.gz",
        "02_clean/{nico}_1.unpaired.fq.gz",
        "02_clean/{nico}_2.paired.fq.gz",
        "02_clean/{nico}_2.unpaired.fq.gz",
    shell:
        "java -jar /software/Trimmomatic-0.36/trimmomatic-0.36.jar PE -threads 16 {input[0]} {input[1]} {output[0]} {output[1]} {output[2]} {output[3]} ILLUMINACLIP:/software/Trimmomatic-0.36/adapters/TruSeq3-PE-2.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36 &"

rule gzip:
    input:
        "02_clean/{nico}_1.paired.fq.gz",
        "02_clean/{nico}_2.paired.fq.gz"
    output:
        "02_clean/{nico}_1.paired.fq",
        "02_clean/{nico}_2.paired.fq"
    run:
        shell("gzip -d {input[0]} > {output[0]}")
        shell("gzip -d {input[1]} > {output[1]}")

rule map:
    input:
        "02_clean/{nico}_1.paired.fq",
        "02_clean/{nico}_2.paired.fq"
    output:
        "03_align/{nico}.sam"
    log:
        "logs/map/{nico}.log"
    threads: 40
    shell:
        "hisat2 -p 20 --dta -x /root/s/r/p/A_th/WT-Al_VS_WT-CK/index/tair10 -1 {input[0]} -2 {input[1]} -S {output} >{log} 2>&1 &"

rule sort2bam:
    input:
        "03_align/{nico}.sam"
    output:
        "03_align/{nico}.bam"
    threads:30
    shell:
        "samtools sort -@ 20 -m 20G -o {output} {input} &"

「rule sort2bam」部分を追加するまで、すべて問題ありません。

ドライランするとうまくいきます。しかし、私がそれを実行すると、質問が説明するようにエラーが報告されます。そして驚いたことに、それはバックグラウンドでスタックしたと報告するタスクを実行します.しかし、常に1つのタスクを実行します.次のように:

rule sort2bam:
    input: 03_align/epcr1.sam
    output: 03_align/epcr1.bam
    jobid: 11
    wildcards: nico=epcr1

Waiting at most 5 seconds for missing files.
MissingOutputException in line 45 of /root/s/r/snakemake/my_rnaseq_data/Snakefile:
Missing files after 5 seconds:
03_align/epcr1.bam
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message

[Sat Apr 27 06:10:22 2019]
rule sort2bam:
    input: 03_align/wt1.sam
    output: 03_align/wt1.bam
    jobid: 9
    wildcards: nico=wt1

Waiting at most 5 seconds for missing files.
MissingOutputException in line 45 of /root/s/r/snakemake/my_rnaseq_data/Snakefile:
Missing files after 5 seconds:
03_align/wt1.bam
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message

[Sat Apr 27 06:23:13 2019]
rule sort2bam:
    input: 03_align/wt2.sam
    output: 03_align/wt2.bam
    jobid: 6
    wildcards: nico=wt2

Waiting at most 5 seconds for missing files.
MissingOutputException in line 44 of /root/s/r/snakemake/my_rnaseq_data/Snakefile:
Missing files after 5 seconds:
03_align/wt2.bam
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message

コードの何が問題なのかわからない 理想はありますか？前もって感謝します！